This guide explains how to load genomes, add TnSeq tracks, navigate, and edit within the
application.
Basics
This application is designed to view bacterial genomes and superimpose data from Tranpose Seq (TnSeq) experiments. To get started first load an annotated bacteiral genome either from the available options or upload a custom genebank file (.gb or .gbb). Once you've added a genome you can then add one or more TnSeq files. These are simple text files with 3 columns, the base number relative to the bacterial chromsome origin, number of inserts in the forward strand, number of inserts in the reverse strand.
Navigating
You can move along the genome via the keyboard or mouse. Use the left or right arrows keys to
move forward or backward through the genome. Holding down shift will 10x the left right movement
speed. Click the bottom navigator to go to any location in the genome or use the search bar to
find a gene by name and click it to center the view around that gene.
←→ Move along genome
Shift + ←/→ 10x move
Click Bottom navigator to jump
Search Find gene by name
Editing
Any gene can be edited by right clicking it. To re-arrange tracks go to the top and hover over the Files button. The currently loaded files will be listed there. You can drag and drop to re-arrange the order of the tracks. You can also click the small trashcan to remove a file or the eye to toggle track visibility. At any time you can drag and drop files from your computer onto the top portion to add more TnSeq data.
